At SNPcode Pvt Ltd, we understand the critical role plasmids play in modern biotechnology. Whether used for cloning, gene therapy, vaccine development, or synthetic biology, plasmids are at the heart of innovation. To ensure their integrity and reliability, plasmid sequencing is essential.

Traditional sequencing methods, like Sanger sequencing, have long been used to verify plasmid constructs. However, these approaches often fall short when plasmids are large, complex, or contain repetitive regions. That’s where Nanopore sequencing comes in—offering an efficient, scalable, and cost-effective solution for complete plasmid sequencing.

Why Choose Nanopore for Plasmid Sequencing?

Nanopore technology, pioneered by Oxford Nanopore Technologies (ONT), is transforming genomics with portable, real-time sequencing devices like the MinION, Flongle, and GridION. For plasmid sequencing, the advantages are clear:

• Complete Coverage in One Read : Long-read sequencing allows entire plasmids—sometimes tens of kilobases long—to be read without fragmentation or assembly challenges.

• Rapid Turnaround : From DNA preparation to sequencing and analysis, results can be obtained within a single day.

• Scalable & Cost-Effective : Multiplexing strategies make it possible to sequence dozens of plasmids simultaneously, lowering per-sample costs.

• Beyond Sequence Verification : Nanopore reads also reveal structural variants and epigenetic modifications, adding an extra layer of insight compared to short-read technologies.

How SNPcode Delivers Nanopore-Based Plasmid Sequencing

At SNPcode, we provide end-to-end plasmid sequencing services powered by Nanopore technology. Our workflow ensures accuracy, reliability, and actionable insights:

1. High-Quality DNA Extraction : We use optimized protocols to obtain intact, high molecular weight plasmid DNA for maximum read length and data quality.

2. Library Preparation & Barcoding : Using ONT’s Rapid Barcoding Kits or Ligation Kits, we prepare libraries tailored to plasmid size and application, enabling multiplexing for efficiency.

3. Real-Time Sequencing : With ONT platforms, data is streamed as it’s generated. Our experts monitor sequencing runs to ensure optimal performance.

4. Advanced Bioinformatics Analysis

   1. Basecalling & Quality Filtering with ONT’s Guppy/Dorado

   2. Assembly & Error Correction using tools like Flye, Canu, or Medaka

   3. Variant Detection & Annotation to confirm inserts, identify point mutations, and validate plasmid features

5. Comprehensive Reporting : We deliver fully assembled plasmid sequences with annotations, highlighting promoters, resistance markers, cloning sites, and any detected mutations.

Applications of Nanopore Plasmid Sequencing

Our clients across academia, biotech, and pharma leverage SNPcode’s Nanopore plasmid sequencing services for:

• Cloning verification – Ensuring constructs match design before downstream use.

• Gene therapy & vaccine development – Verifying plasmid backbones for safety and

  efficacy.

• Synthetic biology – Validating complex modular constructs.

• CRISPR workflows – Confirming gRNA plasmids and donor constructs.

Why Partner with SNPcode Pvt Ltd?

• Expertise: A team experienced in plasmid biology, sequencing, and bioinformatics.

• Customization: Tailored workflows for research, clinical, or industrial needs.

• Reliability: High-accuracy consensus assemblies, even for complex plasmids.

• Speed: Fast turnaround without compromising quality.

Conclusion

With Nanopore sequencing, plasmid validation is no longer a bottleneck—it’s an enabler. At SNPcode Pvt Ltd, we help researchers and biotech innovators move forward with confidence, knowing their plasmids are accurate, intact, and ready for discovery.

If you’re ready to take plasmid sequencing to the next level, connect with SNPcode Pvt Ltd today and explore how our Nanopore-based solutions can accelerate your research and development.